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Research Associate II/Sr Research Associate, Downstream Production & Process Dev

Cambridge, MA 02139

Posted: 11/21/2024 Employment Type: Direct Hire/Perm Job Category: Scientific Job Number: 615942 Is job remote?: No Country: United States

Job Description


Role Overview

We are seeking a passionate Research Associate II/Senior RA to support downstream production and process development of TCR bispecifics and MHC proteins on our Protein Engineering and Molecular Sciences team. The ideal candidate will have hands-on experience in protein purification, with preferred experience refolding proteins from inclusion bodies and/or with chemical modification of proteins. The incumbent will work at the bench to refold, purify, modify and QC TCR bispecifics and MHC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, Octet and analytical methods such as HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner. Interested candidates should have strong organizational, interpersonal, verbal, and written communication skills that will enable them to integrate with our highly cross-functional team.

Key Responsibilities
  • Optimize protein refolding conditions from inclusion bodies.
  • Develop robust processes for protein purification from mammalian expression supernatant or from refolding, using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
  • Execute enzymatic and chemical protein modification protocols.
  • Generate purified and modified proteins at the 5-50 mg scale and maintain inventory.
  • Characterize recombinant proteins using SDS-PAGE, Western blot, protein quantitation, HPLC, ELISA, Octet and other biochemical/biophysical assays to determine protein purity and function.
  • Capture primary data, establish and maintain SOPs, and document work as required in Electronic Laboratory Notebook (ELN), maintaining up to date records.
  • Present data routinely to the Protein Sciences group and other multidisciplinary groups.

Qualifications/Experience
  • BS/MS in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 2+ years of academic or biotech/pharma laboratory experience.
  • Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable.
  • Experience with protein refolding from inclusion bodies and/or protein modification is a plus.
  • Strong organizational and communication skills along with excellent attention to details.
  • Enjoys working in a collaborative, fast-paced and quickly changing environment.

The staffing industry has seen an increase in people falsely representing themselves as recruiters to gather personal information from job seekers. For your safety, do not provide sensitive data to anyone you have not spoken with thoroughly and never provide banking information during the application process. Candidate safety is a top priority at Planet Pharma.

Planet Pharma is an equal opportunity employer. It is our practice not to discriminate against any employee or applicant based on any criteria, condition or basis protected by laws or regulations in the locations where we do business. All qualified applicants are encouraged to apply. We celebrate diversity and are committed to providing an environment of mutual respect. We believe that diversity, equity and inclusion enable us to better meet our mission and values while serving our clients across the globe. If you have a disability or handicap and would like us to accommodate you in any reasonable way, please inform your recruiter, or contact us, so that we can discuss the appropriate alternatives available.

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About Cambridge, MA

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Although this hasn't been an issue at Planet Pharma, the staffing industry has seen an increase in people falsely representing themselves as recruiters to gather personal information from job seekers. For your safety, do not provide sensitive data to anyone you have not spoken with thoroughly and never provide banking information during the application process. Candidate safety is a top priority at Planet Pharma.