Scientist 1 (Protein Expression and Purification, Contract role)
1 DNA Way S San Francisco, CA 94080 US
Job Description
Description The departments of BioMolecular Research and Structural Biology, within the Protein Sciences organization are looking for a versatile, dynamic, self-motivated individual to join us as a Scientist 1. The selected individual will contribute towards recombinant protein expression and purification efforts to support drug discovery activities. This role will facilitate expression analysis of novel protein encoding constructs, by building, developing and utilizing automated high throughput processes for expression, purification and characterization for research and portfolio projects. The qualified candidate must be technically strong, self- driven, passionate about science, results and detail oriented. In this collaborative role, the successful candidate will have the opportunity to work with other scientists and group leaders within and outside our department to fulfill protein production needs. This is an on-site role based in South San Francisco, CA.
Job Responsibilities
- Protein purification using a variety of chromatographic techniques (affinity, size exclusion, ion exchange) for structural and biochemical studies
- Screening and optimizing expression and purification of challenging membrane proteins using detergent lysis
- Using various expression platforms such as E.coli, insect cells (BEVS), and mammalian expression platforms for small and medium scale protein production
- Performing SDS-PAGE, western blot, protein characterization assays by different biophysical and biochemical methods
- Operating and maintaining High performance liquid chromatography systems (HPLC) for protein purification and analysis
- Performing gel analysis, capturing and interpreting data, uploading results to LIMS databases and sharing with stakeholders
- Communicating with and presenting to collaborators and stakeholders across the Protein Sciences organization
- Applicants with a Masters degree in the Biological Sciences should have at least 3-4 years of scientific experience and applicants with a PhD degree (Biochemistry, Biophysics, Cell Biology, Molecular Biology) should have at least 1-2 years of relevant experience
- Strong protein purification expertise in affinity and size exclusion chromatography
- Strong aseptic cell culture techniques for handling routinely insect and mammalian cells
- Ability to think critically, analyze results, plan experiments and carry out troubleshooting as needed
- Strong desire to learn new techniques, advance technologies related to protein optimization and interest in implementing new expression systems.
- Demonstrated ability to work independently in a team-oriented setting
- Expertise with automation and high-throughput process development will be a plus
- Excellent verbal and written communication skills
- Excellent organizational skills
Pay Rate Range: $45-52/hr. Salary will be commensurate with experience
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