Scientist II
200 Sydney St Cambridge, MA 02139 US
Job Description
Target PR Range: 35-45/hr
*Depending on experience
Key Responsibilities:
• Execute high-throughput scRNAseq library preparations by working effectively on a team and leveraging the use of automated liquid handlers (e.g. Biomek i7).
• Operate Illumina sequencers and other lab instrumentation for quality control of NGS Libraries (e.g., Tapestation).
• Accurately document project and sample information, protocols, and experimental details through the Electronic Lab Notebook (ELN)
• Conduct experiments, troubleshoot, and optimize protocols independently and in collaboration with colleagues to deliver high-quality data in a timely fashion.
• Interpret findings and communicate effectively to present scientific data, concepts, and recommendations to various internal audiences.
• Interface with computational biologists and data scientists to generate and interpret scRNAseq datasets.
• Work in matrixed environment and support activities for different groups across the organization, which may include alignment meetings.
Qualifications:
• BS or equivalent education with 3+ years of experience or MS or equivalent education with typically 2+ years of experience.
• Strong background in basic molecular biology and/or genomics with proficiency in molecular biology lab techniques such as DNA/RNA extraction and nucleic acid QC, as well as familiarity with PBMC isolation and handling
Preferred Qualifications:
• Experience with nuclei isolation from PBMCs and/or tissues is preferred.
• Familiarity with 10X Genomics platforms, particularly scRNAseq, Fixed RNA profiling, and/or scATACseq, is preferred.
• Familiarity with automated liquid handlers such as Biomek i7 or Tecan Fluent is a plus but not required.
• Background in R is a plus but not required.
• Background in immunology is a plus but not required.
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