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Scientist II
1000 Gateway Blvd. South San Francisco, CA 94080 US
Job Description
Target PR Range: 50-60/hr
*Depending on experience
Key Responsibilities:
· Design and perform plate-based and cell-based assays (multi-color flow cytometry, Western blot, ELISA, etc.) to assess mRNA delivery and protein expression in mammalian cells using LNP systems.
· Design and perform high-throughput expression and functional screen/characterization assays for novel protein/mRNA candidates, using semi-automated instruments.
· Maintain meticulous records in an electronic lab notebook (ELN), draft standard operating procedures (SOPs), and present results at team meetings.
· Independently plan, execute, and interpret experiments, delivering data-driven insights aligned with project timelines and goals.
· Conduct molecular biology tasks, including cloning (e.g., PCR, golden gate, Gibson reactions, etc.), and optimize mRNA IVT protocols for yield and quality.
· Design, produce, and optimize lentiviral vectors, perform transduction experiments, and analyze results to advance research objectives.
· Stay current with advancements in genetic medicine, LNP technology, and molecular biology techniques to inform experimental design.
· Collaborate with antibody discovery/production teams to support pipeline projects and facilitate technology hand-offs.
Qualifications:
Master’s degree in Cell Biology, Molecular Biology, Biological Sciences, or a related field with 1-3 years of industry experience, OR Bachelor’s degree in a related field with 3+ years of industry experience.
Expertise in mammalian cell culture (suspension and adherent cell lines, primary T cells) and multi-color flow cytometry (panel design, acquisition, and analysis using FlowJo) is required; Hands-on experience with immunological assays (e.g., ELISA, Luminex, cytotoxicity) is highly desirable.
Experience with semi-automated liquid handling systems (e.g., Integra VIAFLO) for high-throughput workflows is highly preferred.
Familiarity with data analysis tools (e.g., GraphPad Prism, FlowJo) is required; working knowledge with statistical and bioinformatics tools (python, R, etc.) is a plus.
Basic understanding of immunology, cancer biology, or cell therapy applications is preferred; Prior experience in genetic medicine, CAR-T cell therapy, or RNA therapeutics is a strong plus.
Basic knowledge of nucleic acid characterization (e.g., gel electrophoresis, qPCR), familiarity with molecular biology techniques, including cloning (e.g., Gibson assembly, PCR) and mRNA IVT (e.g., capping, purification), is a strong plus.
Strong organizational and time-management skills with a proven ability to juggle multiple projects.
Demonstrated ability to troubleshoot assays and optimize protocols independently.
Excellent verbal and written communication skills for presenting data and collaborating with cross-functional teams.
Willingness to take responsibilities outside of original job description.
Equal Opportunity Employer: We are proud to be an equal opportunity employer. We welcome and encourage applications from all qualified candidates regardless of race, sex, gender identity or expression, disability, age, religion or belief, sexual orientation, or any other characteristic protected by applicable laws and regulations. It is our policy not to discriminate against any applicant or employee, and we are committed to fostering a diverse, inclusive, and respectful work environment across all locations in which we operate. We believe that diversity, equity, and inclusion are fundamental to our mission and enhance our ability to serve clients globally. If you have a disability or require any reasonable accommodations during the application or interview process, please inform your recruiter or contact us directly so that we can explore the appropriate arrangements.
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