Senior Research Associate - Molecular Biology
280 UTAH AVE., SUITE 200 SOUTH SAN FRANCISCO, CA 94080 US
Job Description
- Support all tissue culture needed to measure gene editing therapeutics, using both primary and immortalized cell lines
- Perform transfections, including with LNPs, transductions and electroporations of nucleic acids at 96-well scale
- Perform tissue culture assays for measuring gene modification outcomes via NGS and flow cytometry
- Utilize and improve methods for high-throughput tissue culture experiments, including VIAFLO pipettes and liquid-handling robots
- Produce RNA, LNP and virus research articles
- A minimum of 3 years of experience maintaining and transforming multiple immortalized cell lines, and primary cell lines
- Experience with multiple transfection and transduction approaches: ie lipid-based transformations, lonza nucleofectors, and AAV and other viral transductions
- Capable of running and maintaining cytometer and/or high-content imaging instruments, along with preparing samples to run on these instruments
- Experience working with, and developing SOPs for, tissue culture automation tools
- Bachelor's degree in Molecular Biology or related field and 6+ years of experience in the biotech industry, or Master’s degree in Molecular Biology or related field and 4+ years in the biotech industry.
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